Invitrogen™ Phospho-SRC (Tyr530) Monoclonal Antibody (M261)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA547636

Catalog No. PIMA547636

Description

This antibody detects a 60 kDa* protein corresponding to c-Src on SDS-PAGE immunoblots of mouse SYF cells transformed with c-Src and treated with pervanadate. This antibody also detects the conserved site in Lck (Tyr-505) in human Jurkat cells treated with pervanadate. This antibody may detect several c-Src family members phosphorylated at the conserved site for Tyr-530.

SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.

Specifications

• Antigen: Phospho-SRC (Tyr530)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: P05480, P12931, Q9WUD9
• Gene Symbols: SRC
• Immunogen: Clone (M261) was generated from a phosphopeptide containing amino acid surrounding Tyr-530 at the C-terminus of human c-Src. This sequence has high homology to the conserved site in rat and mouse c-Src, and is conserved in the c-Src family of kinases.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Western Blot
• Clone: M261
• Conjugate: Unconjugated
• Gene: SRC
• Gene Alias: ASV; AW259666; csrc; c-SRC; c-src protein, C terminus; EC 2.7.10.2; fc54g04; Neuronal proto-oncogene tyrosine-protein kinase Src; non-tyrosine protein kinase; ORF; OTTHUMP00000174476; OTTHUMP00000174477; OTTHUMP00000174478; p60-Src; p60-Src-1; PP60C-SCR; pp60c-src; pp60src; pp60v-src; protein-tyrosine kinase; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; Proto-oncogene tyrosine-protein kinase Src; Rous sarcoma oncogene; RP5-823N20.1; sb:cb864; SDR; src; src downstream region; src oncogene; SRC proto-oncogene, non-receptor tyrosine kinase; SRC proto-oncogene, non-receptor tyrosine kinase L homeolog; SRC proto-oncogene, non-receptor tyrosine kinase S homeolog; src.L; src.S; SRC1; src-1; src1-A; src-a; src-b; THC6; transforming protein; tyrosine kinase pp60c-src; tyrosine protein kinase c-src; tyrosine protein kinase pp60-c-src; tyrosine-protein kinase SRC-1; Unknown; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma viral oncogene; wu:fc54g04; XELAEV_18046582mg; xsrc
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 20779, 6714, 83805
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid