Invitrogen™ Phospho-STAT1 (Tyr701) Monoclonal Antibody (M135)

Catalog No.	Quantity
PIMA547637	100 μL

Catalog No. PIMA547637 Supplier Invitrogen™ Supplier No. MA547637

Description

Mouse Monoclonal Antibody

The antibody detects 84 and 91 kDa* stat1 variants on SDS-PAGE immunoblots of human A431 treated with EGF, as well as Jurkat and A431 cells treated with pervanadate. The antibody does not detect these variants in control cells.

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.

Specifications

• Antigen: Phospho-STAT1 (Tyr701)
• Applications: Western Blot
• Classification: Monoclonal
• Clone: M135
• Concentration: 0.25 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: STAT1
• Gene Accession No.: P42224, P42225
• Gene Alias: 2010005J02Rik; AA408197; CANDF7; DD6G4-4; DKFZp686B04100; IMD31A; IMD31B; IMD31C; ISGF-3; LOW QUALITY PROTEIN: signal transducer and activator of transcription 1-alpha/beta; OTTHUMP00000165046; OTTHUMP00000205845; signal transducer and activator of transcription 1; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription 1-alpha/beta; signal transducer and activator of transcription-1; STAT; STAT 1; STAT1; STAT-1; stat1 alpha; STAT91; transcription factor ISGF-3; transcription factor ISGF-3 components p91/p84
• Gene Symbols: STAT1
• Host Species: Mouse
• Immunogen: Clone (M135) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 701 of human Stat1. This peptide sequence has high homology to the conserved tyrosine site in rat and mouse stat1.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 20846, 25124, 6772
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1