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Phospho-STAT4 (Tyr693) Monoclonal Antibody (4LURPIE), PE, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

$176.65 - $446.00

Specifications

Antigen Phospho-STAT4 (Tyr693)
Clone 4LURPIE
Concentration 5 μL/Test
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-112-2417
Encompass_Preferred
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Invitrogen™
12904441
25 Tests
Each for $176.65

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Reg : $182.00

The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
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50-112-9226
Encompass_Preferred
View Documents
Invitrogen™
12904442
100 Tests
Each for $446.00
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Add to Cart
 
Description

Description

Description: This 4LURPIE monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 4 (STAT4) when phosphorylated on tyrosine 693 (Y693). STAT proteins are activated by ligand binding to cytokine receptors that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAK proteins, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. STAT4 is activated by IL-12 or type 1 IFN (IFN alpha, IFN beta). STAT4 is required for optimal Th1 differentiation in vivo. Interestingly, although IFN alpha can lead to phosphorylation of STAT4 in mouse T cells, the phosphorylation is much weaker than that induced by IL-12 and is transient. Thus, IFN alpha phosphorylation of STAT4 does not contribute to STAT4-mediated Th1 development or IFN gamma production by Th1 cells in mice. In contrast, IFN alpha does promote Th1 development and function of human CD4+ T cells. Specificity of this 4LURPIE clone was determined by ELISA and flow cytometry. Applications Reported: This 4LURPIE antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 4LURPIE antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test.

STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT1 (52%) and STAT3 (47%). Functionally, STAT4 is similar to other STAT family members in that it can be tyrosine phosphorylated by Jak1 or Jak2. STAT4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT4 can bind the IFN-gamma activated site (GAS). Serine phosphorylation of STAT is also required for maximal transcriptional activity. STAT4 expression is restricted to the thymus, spleen and testis. Until recently the cytokine(s) responsible for activation of STAT4 had not been identified. STAT4 is now known to be activated by the cytokine interleukin 12 (IL-12). IL-12 is required for the T-cell independent induction of IFN-gamma which is a key step in the initial suppression of bacterial and parasitic infections. In addition, IL-12 is required for the development of a Th1 response which is necessary for effective host defense against intracellular pathogens. STAT4-deficient mice display impaired IL-12 development of Th1 cells and enhanced development of Th2 cells. A recent study in mouse has shown that in response to viral infection IFN-a/b activation of STAT4 is required for IFN-g production.
Specifications

Specifications

Phospho-STAT4 (Tyr693)
5 μL/Test
Monoclonal
Liquid
RUO
PBS with BSA and 0.09% sodium azide; pH 7.2
CIS4; HSPC060; OTTHUMP00000163553; Signal transducer and activator of transcription 4; SLEB11; SOCS4; SOCS6; SSI4; STAI4; STAT; Stat4; STATI4
STAT4
Primary
4°C, store in dark, DO NOT FREEZE!
STAT4
4LURPIE
Flow Cytometry
PE
Mouse
Human, Mouse
P42228, Q14765
20849, 6775
IgG1 κ
Affinity chromatography
Antibody
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