Phospho-Stat5 (Tyr694) Monoclonal Antibody (5F6.F1), Rockland™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-194-3791	Rockland Immunochemicals 200301A45	100 μg
50-199-0558	Rockland Immunochemicals 200301A45S	25 μL

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Specific for mouse Stat5a protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5a from other sources has not been determined.

STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

Specifications

• Antigen: Phospho-Stat5 (Tyr694)
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Alias: AA959963; BOS_19069; Mammary gland factor; mammary gland factor STAT5A; MGF; Mpf; signal transducer and activator of transcription 5; Signal transducer and activator of transcription 5A; STAT; STAT5; Stat5a; STAT5A, Mammary Gland Factor; STAT5A/MGF; STAT5B; STATA5
• Gene Symbols: STAT5A
• Isotype: IgG1 κ
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Gene: STAT5A

• Clone: 5F6.F1
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Mouse, Rat
• Gene Accession No.: P42229, P42230, Q62771
• Gene ID (Entrez): 20850, 24918, 6776
• Immunogen: Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
• Primary or Secondary: Primary
• Product Type: Antibody