MilliporeSigma™ phospho-Syk (Tyr323), Rabbit, Alexa Fluor 488, Polyclonal,

Catalog No.	Quantity	Conjugate
ABF1207YMI	100 μL	Alexa Fluor 488

Catalog No. ABF1207YMI Supplier MilliporeSigma™ Supplier No. ABF1207AF488

Description

Rabbit Polyclonal Antibody

60S ribosomal protein L5 (UniProt P46777; also known as RPL5) is encoded by the RPL5 (also known as DBA6, MSTP030) gene (Gene ID 6125) in human. Ribosomal proteins (RPs) are normally involved in the assembly of the translational machinery-ribosomes essential for protein production. RPL5, RPL11 and the 5S rRNA comprise the 5S ribonucleoprotein particle (RNP), an essential subcomplex of the large ribosomal subunit. RPL5 binds the 5S rRNA, the 5S rRNA/RPL5 complex then localizes to the nucleolus, where it binds RPL11 and is integrated into the ribosome. In response to ribosomal stress (RS) or nucleolar stress, the L5/L11/5S pre-ribosomal complex is redirected to inhibit MDM2 E3 ligase activity, thereby promoting p53 stabilization and p53-dependent cell cycle arrest. RPL5 and RPL11 can also bind TAp73 N-terminal TA domain in an RS-independent manner and thereby prevent MDM2 association with TAp73 target gene promoters, resulting in enhanced TAp73 transcriptional activity and TAp73-dependent apoptosis induction and cell cycle arrest.

Specifications

• Antigen: phospho-Syk (Tyr323)
• Applications: Immunocytochemistry
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Alexa Fluor 488
• Formulation: Purified rabbit polyclonal antibody Alexa Fluor™ 488 conjugate in PBS with 1.5% BSA and 0.05% Sodium Azide.
• Gene: SYK
• Gene Accession No.: P43405
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to a sequence surrounding phosphorylated Tyr323 of human Syk.
• Purification Method: Affinity Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_003168
• Test Specificity: Target phosphotyrosine residue coresponds to pTyr323 of human Syk spliced isoform 1 and pTyr300 of human Syk spliced isoform 2. Sequence surrounding the target tyrosine is not well conserved in mouse and rat Syk.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.

For Research Use Only. Not for use in diagnostic procedures.