Invitrogen™ Phospho-Talin (Ser425) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143776

Catalog No. PIPA5143776

Description

The antibody detects a 240 kDa* protein corresponding to the molecular mass of Talin on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.

Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin.

Specifications

• Antigen: Phospho-Talin (Ser425)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TLN2
• Gene Alias: 5730421P04Rik; AI507121; AI787438; AL118320; ILWEQ; KIAA0320; KIAA1027; mKIAA0320; RGD1565416; talin 1; talin 2; talin-1; Talin-2; TLN; Tln1; Tln2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 21894, 313494, 315776, 70549, 7094, 83660
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: P26039, Q71LX4, Q9Y490, Q9Y4G6
• Gene Symbols: TLN1, TLN2
• Immunogen: Phospho-Talin (Ser-425) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-425 in human Talin 1. This sequence is conserved in rat, mouse, and chicken Talin 1, as well as in Talin 2 (Ser-428).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG