Invitrogen™ Phospho-WWTR1 (Ser66) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5105067

Catalog No. PIPA5105067

Description

Antibody detects endogenous levels of TAZ only when phosphorylated at Ser66.

WW domain-containing transcription regulator protein 1 (WWTR1) is a transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor supression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1. In turn, MOB1 phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. WWTR1 regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. WWTR1 also regulates embryonic stem-cell self-renewal and promotes cell proliferation and epithelial-mesenchymal transition.

Specifications

• Antigen: Phospho-WWTR1 (Ser66)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: WWTR1
• Gene Alias: 2310058J06Rik; 2610021I22Rik; C78399; DKFZp586I1419; FLJ27004; FLJ45718; Tafazzin; TAZ; transcriptional coactivator with PDZ binding motif; transcriptional co-activator with PDZ-binding motif; transcriptional coactivator with PDZ-binding motif; transcriptional co-activator with PDZ-binding motif (TA)Z; transcriptional co-activator with PDZ-binding motif (TAZ); WW domain containing transcription regulator 1; WW domain-containing transcription regulator protein 1; Wwtr1
• Host Species: Rabbit
• Purification Method: Sequential chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 25937, 295062, 97064
• Content And Storage: -20°C
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: Q9EPK5, Q9GZV5
• Gene Symbols: WWTR1
• Immunogen: A synthesized peptide derived from human WWTR1(Accession Q9GZV5), corresponding to amino acid residues around phosphorylated Ser66.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov