Invitrogen™ H3K9me3S10ph Recombinant Rabbit Monoclonal Antibody (RM162)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA533083

Catalog No. PIMA533083

Description

Specificity: reacts to Histone H3 only when modified AT both trimethyl lysine 9 and phospho serine 10 (K9me3/S10p). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specifications

• Antigen: H3K9me3S10ph
• Classification: Recombinant Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 1% BSA, 50% glycerol and 0.09% sodium azide
• Gene Accession No.: P68431, P68433, P84228, Q71DI3
• Gene Symbols: H3C1, H3C10, H3C12, H3C14, H3C15, H3C2, H3C7, Hist1h2ail1
• Immunogen: A trimethyl-phospho-peptide corresponding to Trimethyl- Phospho-Histone H3 (Lys9/Ser10).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, All species
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Western Blot, Luminex
• Clone: RM162
• Conjugate: Unconjugated
• Gene: H3C15
• Gene Alias: H3; H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 histone, family 3A; H3 histone, family 3B; H3 histone, family 3B (H3.3B); H3 histone, family 3C; H3 K9; H3 K9me3; H3 S10; H3 S10ph; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; H3.3A; H3.3b; H3.5; H3/A; H3/b; H3/d; H3/f; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3-143; H3-291; H3-3A; H3-3B; H3-53; H3-614; H3a; H3b; H3-B; H3c1; H3c10; H3c11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3c8; H3f; H3-F; H3F1K; H3F2; H3F3; H3F3A; H3F3B; H3F3C; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; H3K9; H3K9me3; H3K9me3S10p; H3K9trS28p; H3K9trS28ph; H3Lys9me3Ser10p; H3Lys9me3Ser10ph; H3Lys9trSer28p; H3Lys9trSer28ph; H3s10; H3S10ph; Hist1; Hist1h2ai; Hist1h2ail; Hist1h2ail1; Hist1h3a; HIST1H3B; Hist1h3c; HIST1H3D; HIST1H3E; HIST1H3F; Hist1h3g; HIST1H3H; Hist1h3i; HIST1H3J; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; histone 1, H2ai; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 1, H2ai; histone cluster 1, H2ai-like; histone cluster 1, H2ai-like1; histone cluster 1, H3a; histone cluster 1, H3b; histone cluster 1, H3f; histone cluster 1, H3h; histone cluster 2, H3a; histone cluster 2, H3c; histone cluster 2, H3c2; histone cluster 2, H3c2, pseudogene; histone gene complex 1; histone H3; Histone H3 K9; Histone H3 K9me3; Histone H3 S10; Histone H3 S10ph; histone H3.1; Histone H3.2; histone H3.3; Histone H3.3C; histone H3.5; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; histone H3/l; Histone H3/m; histone H3/o; Histone H3K9; Histone H3K9me3; Histone H3K9me3S10ph; Histone H3S10; Histone H3S10ph; histone variant H3.5; M32461; methyl Histone 3; methyl Histone H3; phospho Histone 3; phospho Histone H3; Phospho-Tri-Methyl-H3 K9 S10; Phospho-Tri-Methyl-H3 Lys9 Ser10; Phospho-Tri-Methyl-Histone H3 K9 S10; Phospho-Tri-Methyl-Histone H3 Lys9 Ser10; Phospho-Tri-Methyl-Histone H3 S10 K9; Phospho-Tri-Methyl-Histone H3 S28 K27; Phospho-Tri-Methyl-Histone H3 Ser10 Lys9; Phospho-Tri-Methyl-Histone H3 Ser28 Lys27; PP781; Trimethyl H3; Tri-methyl H3; Trimethyl Histone H3; Tri-methyl Histone H3
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 126961, 260423, 291159, 319150, 319152, 333932, 360198, 8350, 8356, 8357, 8358, 8968, 97114
• Content And Storage: -20°C
• Form: Liquid