Invitrogen™ Phospho-TRPM7 (Ser1493) Polyclonal Antibody

Catalog No.	Quantity
PIPA5143778	100 μL

Catalog No. PIPA5143778 Supplier Invitrogen™ Supplier No. PA5143778

Description

Rabbit Polyclonal Antibody

The antibody was cross-adsorbed to unphosphorylated TRPM7 (Ser-1493) then affinity purified using phospho-TRPM7 (Ser-1493) peptide. This antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 cells stimulated with calyculin A, and detects a 70 kDa autophosphorylated recombinant kinase domain of human TRPM7. These reactivities are not observed after lambda phosphatase treatment to dephosphorylate TRPM7.

TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.

Specifications

• Antigen: Phospho-TRPM7 (Ser1493)
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1 mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: Trpm7
• Gene Accession No.: Q96QT4
• Gene Alias: 2310022G15Rik; 4833414K03Rik; 5033407O22Rik; ALSPDC; CHAK; CHAK1; Channel kinase 1; Channel-kinase 1; FLJ20117; long transient receptor potential channel 7; long transient receptor potential channel 7 {ECO:0000250; Ltpr7; LTRPC ion channel family member 7; LTRPC7; LTrpC-7; LTrpC7 {ECO:0000250; PubMed:11161216}; RGD:620053}; tr; transient receptor potential cation channel subfamily M member 7; transient receptor potential cation channel subfamily M member 7 {ECO:0000312; transient receptor potential cation channel, subfamily M, member 7; transient receptor potential M7; transient receptor potential-phospholipase C-interacting kinase; transient receptor potential-phospholipase C-interacting kinase {ECO:0000303; transient receptor potential-related protein, ChaK; TRMP7; TRP PLIK; Trpm7; trpm7 {ECO:0000312; TRPPLIK; Trp-plik; TRP-PLIK {ECO:0000303; UniProtKB:Q96QT4}
• Gene Symbols: Trpm7
• Host Species: Rabbit
• Immunogen: Phospho-TRPM7 (Ser-1493) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1493 in human TRPM7. This site is conserved in rat and mouse TRPM7, but the homology surrounding the site is low. This site has no homology to other TRPM family members.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 54822
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG