Invitrogen™ Phospho-TPH1 (Ser58) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA14644

Catalog No. PA14644

Description

This antibody is predicted to react with bovine, chicken, human, mouse, non-human primate, Xenopus, rat and zebrafish based on 100% sequence homology. This antibody is specific for the ~55 kDa Tryptophan Hydroxylase protein phosphorylated at Ser58 in Western blots of human dorsal Raphe nucleus.

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).

Specifications

• Antigen: Phospho-TPH1 (Ser58)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TPH1
• Gene Alias: indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; TPH; Tph1; TPH2; TPRH; TRPH; tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase); tryptophan hydroxylase 1; tryptophan hydroxylase 1 (tryptophan 5-monooxygenase)
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100009100, 100101558, 21990, 24848
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.3 mg/mL
• Formulation: 0.01M HEPES with 100μg/mL BSA, 50% glycerol, 0.15M NaCl and no preservative; pH 7.5
• Gene Accession No.: P09810, P17290, P17532
• Gene Symbols: TPH1
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 of rat TPH1 conjugated to KLH.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Mouse, Rat, Rabbit
• Product Type: Antibody
• Isotype: IgG