Phospho-Tyrosine Antibody, R&D Systems™

Mouse Monoclonal Antibody

Supplier:  R&D Systems MAB1676500

Catalog No. MAB1676500

Description

Phospho-Tyrosine Monoclonal specifically detects Phospho-Tyrosine in Multi-Species samples. It is validated for Western Blot, Simple Western, Immunoprecipitation, Immunocytochemistry.

Specifications

• Antigen: Phospho-Tyrosine
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Host Species: Mouse
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO
• Test Specificity: Detects proteins containing phosphorylated tyrosine residues. ELISA and Western blot analyses using pervanadate-treated cell lysates indicate that clone 179003 binds phospho-tyrosine in a broad manner largely independent of the surrounding amino acid sequence. No cross-reactivity with proteins or peptides containing phosphorylated serine or threonine residues is observed.
• Target Species: Multi-species
• Form: Supernatant

• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry
• Clone: 179003
• Dilution: Western Blot 1 ug/mL, Simple Western 50 ug/mL, Immunoprecipitation 5 ug/500 ug cell lysate, Immunocytochemistry 8-25 ug/mL
• Gene Alias: 2 amino 3(4 hydroxyphenyl) propanoic acid, 4 hydroxyphenylalanine, PhosphoTyrosine, pTyrosine, Tyrosine
• Immunogen: KLH-coupled phospho-tyrosine synthetic peptide
• Quantity: 500 μg
• Primary or Secondary: Primary
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Isotype: IgG1

For Research Use Only