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Phospho-Tyrosine Antibody, R&D Systems™
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Catalog No. MAB1676SP
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25 μg
500 μg
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Catalog No. Quantity
MAB1676SP 25 μg
MAB1676100 100 μg
MAB1676500 500 μg
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Catalog No. MAB1676SP Supplier R&D Systems Supplier No. MAB1676SP
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Description

Mouse Monoclonal Antibody

Phospho-Tyrosine Monoclonal specifically detects Phospho-Tyrosine in Multi-Species samples. It is validated for Western Blot, Simple Western, Immunoprecipitation, Immunocytochemistry.

Specifications

Antigen Phospho-Tyrosine
Applications Western Blot, Immunoprecipitation, Immunocytochemistry
Classification Monoclonal
Clone 179003
Conjugate Unconjugated
Dilution Western Blot 1 ug/mL, Simple Western 50 ug/mL, Immunoprecipitation 5 ug/500 ug cell lysate, Immunocytochemistry 8-25 ug/mL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Alias 2 amino 3(4 hydroxyphenyl) propanoic acid, 4 hydroxyphenylalanine, PhosphoTyrosine, pTyrosine, Tyrosine
Host Species Mouse
Immunogen KLH-coupled phospho-tyrosine synthetic peptide
Purification Method Protein A or G purified from hybridoma culture supernatant
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Test Specificity Detects proteins containing phosphorylated tyrosine residues. ELISA and Western blot analyses using pervanadate-treated cell lysates indicate that clone 179003 binds phospho-tyrosine in a broad manner largely independent of the surrounding amino acid sequence. No cross-reactivity with proteins or peptides containing phosphorylated serine or threonine residues is observed.
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Target Species Multi-species
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Form Purified
Isotype IgG1
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