MilliporeSigma™ phospho-Ubiquitin (Ser65), Rabbit, Alexa Fluor 488, Polyclonal,

Catalog No.	Quantity	Conjugate
ABS1513IYMI	100 μL	Alexa Fluor 488

Catalog No. ABS1513IYMI Supplier MilliporeSigma™ Supplier No. ABS1513IAF488

Description

Rabbit Polyclonal Antibody

Lysine-specific demethylase 6A (UniProt O15550; also known as Histone demethylase UTX, KDM6A, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein) is encoded by the KDM6A (also known as KABUK2, UTX) gene (Gene ID 7403) in human. KDM6A specifically demethylates Lys-27 of histone H3 and plays a central role in histone code. It is shown to demethylate trimethylated and dimethylated, but not monomethylated H3 Lys-27. KDM6A plays a vital role in regulation of posterior development, by regulating HOX gene expression. KDM6A also plays a potential role in regulating brown adipocyte thermogenic program. It is up-regulated during brown adipocyte differentiation and by cold exposure in both brown and white adipose tissue in mice, indicating its potential role in thermogenesis. Inactivation of KDM6A down-regulates brown fat specific gene expression. Mutations in KDM6A gene are a causative factor in Kabuki syndrome that is characterized by congenital mental retardation, postnatal dwarfism, large prominent earlobes, a cleft or high-arched palate, scoliosis, and other abnormalities.

Specifications

• Antigen: phospho-Ubiquitin (Ser65)
• Applications: Immunocytochemistry
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Alexa Fluor 488
• Formulation: Purified rabbit polyclonal antibody in PBS with 1.5% BSA and 0.05% Sodium Azide.
• Gene: UBB, UBC
• Gene Accession No.: P0CG47;P0CG478
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to the ubiquitin target region sequence containing phosphorylated Ser65.
• Purification Method: Affinity Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_061828;NP_066289
• Test Specificity: Target specificity was verified using the unconjugated antibody (ABS1513-I). Only Ser65-phosphorylated ubiquitin (Ub) and ubiquitinated proteins (including oligoUb and polyUb) containing Ser65-phosphorylated ubiquitin were detected. This antibody exhibits no reactivity toward Ub or ubiquitinated proteins in the absence of Ub Ser65 phosphorylation.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified

For Research Use Only. Not for use in diagnostic procedures.