Invitrogen™ Phospho-ULK1 (Ser555) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA578249

Catalog No. PIPA578249

Description

Positive Control: MCF-7, MCF-7 (100 μM CCCP treatment for 2 hr) Predicted Reactivity: Mouse (100%), Rat (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

Specifications

• Antigen: Phospho-ULK1 (Ser555)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ULK1
• Gene Alias: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; ATG1A; AU041434; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; hATG1; KIAA0722; mKIAA0722; Serine/threonine-protein kinase ULK1; serine/threonine-protein kinase Unc51.1; ULK 1; Ulk1; UNC 51; UNC51; unc-51 like autophagy activating kinase 1; unc-51 like kinase 1; Unc51.1; unc-51-like kinase 1
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 8408
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.13 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: O75385
• Gene Symbols: ULK1
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ULK1 (phospho Ser555). The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov