Invitrogen™ Phospho-ULK1 (Ser758) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5105130

Catalog No. PIPA5105130

Description

Antibody detects endogenous levels of ULK1 only when phosphorylated at Ser757 of mouse ULK1 or Ser758 of human ULK1.

ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

Specifications

• Antigen: Phospho-ULK1 (Ser758)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ULK1
• Gene Alias: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; ATG1A; AU041434; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; hATG1; KIAA0722; mKIAA0722; Serine/threonine-protein kinase ULK1; serine/threonine-protein kinase Unc51.1; ULK 1; Ulk1; UNC 51; UNC51; unc-51 like autophagy activating kinase 1; unc-51 like kinase 1; Unc51.1; unc-51-like kinase 1
• Host Species: Rabbit
• Purification Method: Sequential Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22241, 360827, 8408
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: O70405, O75385
• Gene Symbols: ULK1
• Immunogen: A synthesized peptide derived from mouse ULK1(Accession O75385), corresponding to amino acid residues around phosphorylated Ser758.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov