MilliporeSigma™ phospho-VE-Cadherin (Tyr685), Rabbit, Alexa Fluor 555, Polyclonal,

Catalog No.	Quantity	Conjugate
ABT1760ALMI	100 μL	Alexa Fluor 555

Catalog No. ABT1760ALMI Supplier MilliporeSigma™ Supplier No. ABT1760AF555

Description

Rabbit Polyclonal Antibody

Cadherin-5 (UniProt P33151; also known as 7B4 antigen, CD144, Vascular endothelial cadherin, VE-Cad, VE-cadherin) is encoded by the CDH5 gene (Gene ID 1003) in human. Cadherins (calcium-dependent adhesion) are type-1 transmembrane proteins that form adherens junctions in tissues and play important roles in mediating cell adhesion. Cadherins are designated with a prefix that specifies their tissue association. Vascular endothelial-cadherin (VE-cadherin) is the transmembrane component of the endothelial adherens junction between vascular endothelial cells (ECs) and plays a pivotal role in endothelium integrity and in the control of vascular permeability. One characteristic of VEGF-induced vascular permeability is the phosphorylation of VE-cadherin by Src family kinases (SFK), which leads to VE-cadherin internalization and the destabilization of adherens junctions. Likewise, VE-cadherin overexpression is shown to decrease the permeability of endothelial monolayers in vitro. SFK activation by dominant negative Csk overexpression is reported to result in VE-cadherin phosphorylation at tyrosines 658, 685, and 731 in human dermal microvascular endothelial cells (HDMECs) without affecting HDMEC monolayer permeability. On the other hand, expression of constitutively active Src promoted VE-cadherin phosphorylation on tyrosines 658 and 731 and decreased barrier function, suggesting that concurrent signaling events in addition to VE-cadherin tyrosine phosphorylation are needed to promote barrier permeability in response to inflammatory mediators or growth factors. VE-cadherin is initially produced with a signal peptide (a.a. 1-24) and a propeptide (a.a. 25-45) sequence, the removal of which yields tthe mature protein containing a large extracellular region (a.a. 46-599) with five cadherine repeats (a.a. 46-149, 150-256, 257-371, 372-476, 477-593), followed by a transmembrane segment (a.a. 600-620) and a cytoplasmic domain (a.a. 621-784).

Specifications

• Antigen: phospho-VE-Cadherin (Tyr685)
• Applications: Immunocytochemistry
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Alexa Fluor 555
• Formulation: Purified rabbit polyclonal antibody Alexa Fluor™ 555 conjugate in PBS with 1.5% BSA and 0.05% Sodium Azide.
• Gene: CDH5
• Gene Accession No.: P33151
• Host Species: Rabbit
• Immunogen: KLH-conjugated linear peptide corresponding to a sequence surrounding phosphorylated Tyr685 of human VE-cadherin isoform 1 precursor sequence.
• Purification Method: Affinity Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001786
• Test Specificity: Target specificity of the unconjugated antibody (Mfr. No. ABT1760) has been verified by immunogen peptide blocking in Western blotting application. Numbering of the target VE-cadherin phosphotyrosine residue varies depending on whether the N-terminal signal peptide sequence and propeptide sequence are included, as well as the species and spliced isoform a particular numbering is based on. Target pTyr this rabbit polyclonal detects corresponds to pTyr685/pTyr638 of human spliced isoform 1 precursor/mature sequence (UniProt P33151-1), pTyr570/pTyr523 of human spliced isoform 2 precursor/mature sequence (UniProt P33151-2), pTyr685/pTyr640 of bovine precursor/mature sequence (UniProt Q6URK6), and pTyr684/pTyr40 of porcine precursor/mature sequence (UniProt O02840). Equivalent target site sequence is not present in mouse, rat, or chicken VE-cadherin sequence.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified

For Research Use Only. Not for use in diagnostic procedures.