Invitrogen™ Phospho-Vinculin (Tyr1065) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 441078G

Catalog No. 44-107-8G

Description

Phospho-Vinculin (Tyr1065) Polyclonal Antibody for Western Blot, ICC/IF

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

Specifications

• Antigen: Phospho-Vinculin (Tyr1065)
• Classification: Polyclonal
• Formulation: Dulbecco's PBS with 1 mg/mL BSA and 0.05% sodium azide; pH 7.3
• Gene Accession No.: P12003, P18206
• Gene Symbols: VCL
• Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 1065. The sequence is conserved in mouse and chicken.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Chicken
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Gene: VCL
• Gene Alias: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; epididymis luminal protein 114; HEL114; metavinculin; meta-vinculin; metavinculin {ECO:0000250; MV; MVCL; RP11-178G16.3; UniProtKB:P18206}; unnamed protein product; Vcl; vcl {ECO:0000250; VIN; vinc; vinc 1; VINC1; Vinculin; vinculin {ECO:0000250
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 396422, 7414
• Content And Storage: -20°C
• Form: Liquid