Invitrogen™ Phosphoserine/threonine Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5121314

Catalog No. PIPA5121314

Description

Positive test controls include: C6.

A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine. RSK1, p70 S6 K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity. The (Ser/Thr) Phe Antibody is a powerful tool for discovery of new proteins containing this important regulatory motif.

Specifications

• Antigen: Phosphoserine/threonine
• Classification: Polyclonal
• Conjugate: Unconjugated
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Target Species: Chemical, Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Western Blot
• Concentration: 2.95 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.3
• Immunogen: A synthetic peptide corresponding to a sequence containing phosphorylated S & T.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid
• Target Molecule: Chemical