Invitrogen™ Phosphotyrosine Monoclonal Antibody (P-Tyr-01)

Catalog No.	Quantity
PIMA110443	100 μg

Catalog No. PIMA110443 Supplier Invitrogen™ Supplier No. MA110443

Description

Mouse Monoclonal Antibody

This antibody detects tyrosine phosphorylation in activated cells.

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

Specifications

• Antigen: Phosphotyrosine
• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: P-Tyr-01
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Alias: Phosphotyrosine; pTyr; pY
• Host Species: Mouse
• Immunogen: Phosphotyrosine conjugated to bovine serum albumin.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Chemical
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1
• Target Molecule: Chemical