Invitrogen™ Phosphotyrosine Monoclonal Antibody (pY20), Biotin

Catalog No.	Quantity
01-674-306	100 μg

Catalog No. 01-674-306 Supplier Invitrogen™ Supplier No. MA112439

Description

Mouse Monoclonal Antibody

Phosphotyrosine Monoclonal Antibody for ELISA, ICC/IF, IHC, IP, Western Blot

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

Specifications

• Antigen: Phosphotyrosine
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: pY20
• Concentration: 1 mg/mL
• Conjugate: Biotin
• Formulation: PBS with 65% glycerol and 0.08% sodium azide
• Gene Alias: Phosphotyrosine; pTyr; pY
• Host Species: Mouse
• Immunogen: Phosphotyrosine coupled to carrier protien.
• Purification Method: Protein A/G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Chemical
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b
• Target Molecule: Chemical