Invitrogen™ Phosphotyrosine Monoclonal Antibody (pY20), FITC

Catalog No.	Quantity
01-674-308	100 μg

Catalog No. 01-674-308 Supplier Invitrogen™ Supplier No. MA112441

Description

Mouse Monoclonal Antibody

This antibody does not react with phosphorylated threonine or serine residues.

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

Specifications

• Antigen: Phosphotyrosine
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: pY20
• Concentration: 1 mg/mL
• Conjugate: FITC
• Formulation: PBS with 50% glycerol and 3mM sodium azide; pH 7.5
• Gene Alias: Phosphotyrosine; pTyr; pY
• Host Species: Mouse
• Immunogen: Phosphotyrosine coupled to carrier protien.
• Purification Method: Protein A/G
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Chemical
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles, store in dark
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b
• Target Molecule: Chemical