Invitrogen™ Phosphotyrosine Monoclonal Antibody (pY20), HRP

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA112445

Catalog No. 01-674-312

Description

A suggested positive control for this product is A431 cells stimulated with EGF.

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

Specifications

• Antigen: Phosphotyrosine
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 60% glycerol and 0.1% chloroacetamide
• Host Species: Mouse
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Clone: pY20
• Conjugate: HRP
• Gene Alias: Phosphotyrosine; pTyr; pY
• Immunogen: Phosphotyrosine coupled to carrier protein.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid
• Target Molecule: Chemical