Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

MilliporeSigma™ Phosphotyrosine, recombinant, Mouse, Alexa Fluor 647, Clone: 4G10,

Mouse Monoclonal Antibody

Manufacturer:  MilliporeSigma™ 05777AF647

Catalog No. 05777AF647


Add to cart

Description

Description

Phosphorylation plays an important role in regulating protein activities and various cellular signaling events. Posttranslational phosphorylation can occur on histidine (pHis), serine (pSer), threonine (pThr), and tyrosine (pTyr) residues. Due to the early advent of anti-pTyr antibodies, tyrosine phosphorylation remains the mostly studied signaling events in all biological research fields. Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Before the availability of anti-pTyr antibodies, protein tyrosine phosphorylation was routiniely detected by time-consuming radioactive labeling. Although radioactive labeling is still considered as more sensitive, advancement in detection technology has greatly improved the detection limits of antibody-based methods. Anti-pTyr antibodies are commonly used in ELISA, immunoprecipitation, immunoblotting (including dot blot and Western blot), as well as indirect immunofluourescent detection of tyrosine phosphorylation in tissue and cell samples.
Specifications

Specifications

Phosphotyrosine, recombinant
4G10
Alexa Fluor 647
Purified
Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Purified via heavy chain C-terminus hexa-histidine tag by Nickel affinity matrices prior to conjugation with Alexa Fluor™ 647.
100μL
Signaling
Primary
Recognizes tyrosine-phosphorylated proteins from all species.
Immunocytochemistry
Please refer to lot specific datasheet.
Purified mouse monoclonal IgG2a antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % Sodium Azide.
Mouse
Protein G Purified
RUO
Stable for 1 year at 2°-8°C from date of receipt.
Monoclonal
All
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.