MilliporeSigma™ Phosphotyrosine, recombinant, Mouse, Alexa Fluor 647, Clone: 4G10,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ 05777AF647

Catalog No. 05777AF647

Description

Phosphorylation plays an important role in regulating protein activities and various cellular signaling events. Posttranslational phosphorylation can occur on histidine (pHis), serine (pSer), threonine (pThr), and tyrosine (pTyr) residues. Due to the early advent of anti-pTyr antibodies, tyrosine phosphorylation remains the mostly studied signaling events in all biological research fields. Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Before the availability of anti-pTyr antibodies, protein tyrosine phosphorylation was routiniely detected by time-consuming radioactive labeling. Although radioactive labeling is still considered as more sensitive, advancement in detection technology has greatly improved the detection limits of antibody-based methods. Anti-pTyr antibodies are commonly used in ELISA, immunoprecipitation, immunoblotting (including dot blot and Western blot), as well as indirect immunofluourescent detection of tyrosine phosphorylation in tissue and cell samples.

Specifications

• Antigen: Phosphotyrosine, recombinant
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified mouse monoclonal IgG2a antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % Sodium Azide.
• Immunogen: Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Purified via heavy chain C-terminus hexa-histidine tag by Nickel affinity matrices prior to conjugation with Alexa Fluor™ 647.
• Quantity: 100 μL
• Research Discipline: Signaling
• Test Specificity: Recognizes tyrosine-phosphorylated proteins from all species.
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.

• Applications: Immunocytochemistry
• Clone: 4G10
• Conjugate: Alexa Fluor 647
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: All species
• Form: Purified

For Research Use Only. Not for use in diagnostic procedures.