Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Molecular Probes™ pHrodo™ Green STP Ester Non-distribution product as customer accommodation.

pHrodo™ Green STP Ester

Manufacturer:  Molecular Probes™ P35369

Catalog No. P35369


Add to cart

Description

Description

The amine-reactive pH-sensitive pHrodo™ Green STP ester dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo™ Green dramatically increases fluorescence as the pH of its surroundings become more acidic.
  • Use pH-sensitive pHrodo™ Green STP ester to make pH-sensitive bioconjugates of your choice
  • Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps or quenchers
  • Multiplex with red fluorescent markers such as RFP, pHrodo™ Red, and many others

    The increase in fluorescence of pHrodo™ Green as pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it an ideal tool to study endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo™ Green makes it useful for multi-color experiments. pHrodo™ Green can be detected using most standard green fluorescent filter sets and has been validated for use on a variety of platforms, including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo™ Green in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.

    The pHrodo™ Green STP ester is an amine reactive dye that can be used to make pHrodo™ Green bioconjugates in aqueous buffer. The STP ester will react with primary amines on a protein, cell, or virus to create a stable conjugate that can be used in live cell assays or stored for later use.

    Amine reactive pHrodo™ dye is also available with red fluorescence (see pHrodo™ Red SE). In addition, thiol-reactive pHrodo™ Red Maleimide and Green Maleimide are a great alternative to amine-reactive dyes for antibody labeling. Thiol labeling ensures that the dye will not attach to the binding region of the antibody. pHrodo™ Green and Red conjugates, for example dextran, E. coli and S. aureus, are also available in ready-to-use form.

    For Research Use Only. Not for human or animal therapeutic or diagnostic use.
  • Specifications

    Specifications

    Amine
    Fluorescent
    505⁄525
    Room Temperature
    pHrodo™ Green
    DMSO (Dimethylsulfoxide)
    1 vial , store ≤ -20 °C, dessicate and protected from light.
    75000
    STP Ester
    Green
    pHrodo™
    SDS
    Documents
    Provide Content Correction

    The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

    Product Title

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

    Cancel Submit

    For Research Use Only. Not for use in diagnostic procedures.