Molecular Probes pHrodo Green STP Ester
pHrodo Green STP Ester
Manufacturer: Molecular Probes P35369
DescriptionThe amine-reactive pH-sensitive pHrodo Green STP ester dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo Green dramatically increases fluorescence as the pH of its surroundings become more acidic.
The increase in fluorescence of pHrodo Green as pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it an ideal tool to study endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo Green makes it useful for multi-color experiments. pHrodo Green can be detected using most standard green fluorescent filter sets and has been validated for use on a variety of platforms, including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo Green in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.
The pHrodo Green STP ester is an amine reactive dye that can be used to make pHrodo Green bioconjugates in aqueous buffer. The STP ester will react with primary amines on a protein, cell, or virus to create a stable conjugate that can be used in live cell assays or stored for later use.
Amine reactive pHrodo dye is also available with red fluorescence (see pHrodo Red SE). In addition, thiol-reactive pHrodo Red Maleimide and Green Maleimide are a great alternative to amine-reactive dyes for antibody labeling. Thiol labeling ensures that the dye will not attach to the binding region of the antibody. pHrodo Green and Red conjugates, for example dextran, E. coli and S. aureus, are also available in ready-to-use form.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
|1 vial , store ≤ -20 °C, dessicate and protected from light.|
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For Research Use Only. Not for use in diagnostic procedures.