Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

Catalog Number	Mfr. No.	No. of Reactions
F548S	Thermo Scientific™ F548S	100 Reactions
F548L	Thermo Scientific™ F548L	500 Reactions

Description

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix preserves the fidelity and the yield in reactions when using very short PCR protocols. The unique master mix composition permits usage of short cycling protocols with both low and high complexity DNA templates (15 seconds per kilobase or less). The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase.

Features

• Extreme speed with extension times of 15 s/kb or less
• Hot start modification allows 'zero-time reactivation'
• Accurate proofreading DNA polymerase with a fidelity of 25X Taq
• High yields in reduced cycling time
• Minimized pipetting steps

Applications

• Fast PCR
• High-fidelity PCR
• Difficult (GC-rich) templates
• Template generation for sequencing Multiplex PCR
• Long-range PCR
• Cloning & mutagenesis
• Microarray

Notes

• Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifications

• Concentration: 2X
• Polymerase: Phusion Flash II DNA Polymerase
• Product Type: High-Fidelity PCR Master Mix
• For Use With (Application): Hot-start PCR, High-fidelity PCR
• Hot Start: Built-In Hot Start
• Reaction Format: SuperMix or Master Mix
• Color: Colorless

• GC-Rich PCR Performance: High
• Reaction Speed: Fast
• Shipping Condition: Dry Ice
• Fidelity (vs. Taq): 25X
• Overhang: Blunt
• Size (Final Product): 20 kb or less

For Research Use Only. Not for use in diagnostic procedures.