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Thermo Scientific™ Phusion High-Fidelity PCR Kit

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

Manufacturer:  Thermo Scientific™ F553L

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Catalog No. F553L

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  • Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
  • Polymerase speed allows short extension times (15 to 30 s/kb)
  • Robust performance, minimal optimization needed
  • Increased product yields with minimal enzyme amounts
  • Direct loading on gel with clear Buffer


Phusion High-Fidelity DNA Polymerase
20 kb
PCR Amplification
200 x 50µL Rxns (500 x 20µL Rxns)
52 X
Separate Components
200 Reactions
High-Fidelity PCR Kit
Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS
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