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Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with GC Buffer

The Thermo Scientific™ Phusion™ High-Fidelity PCR Master Mixes are high performance enzymes now available in convenient master mix format.

$199.65 - $1003.00

Specifications

Format Tube
GC-Rich PCR Performance High
Polymerase Phusion High-Fidelity DNA Polymerase
Reaction Speed Fast
Product Line Phusion
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Products 2
Catalog Number Mfr. No. Sufficient For Price Quantity & Availability  
Catalog Number Mfr. No. Sufficient For Price Quantity & Availability  
F532L
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Thermo Scientific™
F532L
500 x 50 μL Reactions
Each for $1,003.00
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F532S
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Thermo Scientific™
F532S
100 x 50 μL Reactions
Each for $199.65

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Reg : $251.00

The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
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Description

Description

The Thermo Scientific™ Phusion™ High-Fidelity PCR Master Mixes are high performance enzymes now available in convenient master mix format.

Phusion High-Fidelity PCR Master Mix with HF Buffer and Phusion High-Fidelity PCR Master Mix with GC Buffer are convenient 2x mixes containing Phusion DNA Polymerase, nucleotides and optimized HF or GC reaction buffer including MgCl2. Only template and primers need to be added by the user.
  • High fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
  • Speed of the polymerase allows short extension times (15-30 s/kb)
  • Robust reactions, minimal optimization needed
  • Increased product yields with minimal enzyme amounts

Includes:

2x Phusion High-Fidelity PCR Master Mix with HF Buffer contains Phusion DNA Polymerase, 2x Phusion HF Buffer and 400μM of each dNTP. The master mix supplies 1.5 mM MgCl2 and 200μM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product. 2x Phusion High-Fidelity PCR Master Mix with GC Buffer contains Phusion DNA Polymerase, 2x Phusion GC Buffer and 400μM of each dNTP. The master mix supplies 1.5 mM MgCl2 and 200μM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product.

Recommended for:

Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray

Specifications

Specifications

Tube
Phusion High-Fidelity DNA Polymerase
Phusion
DHPLC
2X
No
SuperMix or Master Mix
DNA
High
Fast
High-Fidelity PCR Master Mix
Cloning
25 X
Blunt
≤20 kb
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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