Invitrogen™ Phycoerythrin Monoclonal Antibody (eBioPE-DLF), Biotin, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-9375	Invitrogen™ 13412082	100 μg

Description

Description: The eBioPE-DLF monoclonal antibody reacts with phycoerythrin (PE), PE-Cy5 and PE-Cy7, with no crossreactivity to fluorescein (FITC) or allophycocyanin (APC). When using eBioPE-DLF as a second-step antibody, in combination with an appropriate third step, to bind a PE-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated PE antibody. Therefore no interference or quenching of the PE fluorochrome is observed. The eBioPE-DLF can be used for separation of cells labeled with PE-conjugated antibodies or staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary PE-conjugated antibody and the application tested. Applications Reported: This eBioPE-DLF antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioPE-DLF antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

Anti-RPE (R-Phycoerythrin) antibodies can be used to bind conjugated primary antibody without decreasing fluorescent intensity of the RPE fluorochrome. This method is used for separation of cells labeled with RPE-conjugated antibodies.

Specifications

• Antigen: Phycoerythrin
• Concentration: 0.5 mg/mL
• Applications: Flow Cytometry
• Conjugate: Biotin
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: eBioPE-DLF
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG1 κ
• Target Species: Chemical
• Gene Alias: PE; RPE; R-PE; R-phycoerythrin
• Product Type: Antibody