MilliporeSigma™ PI3 Kinase p85, N-SH2 domain, Rabbit, Alexa Fluor 488, Polyclonal,

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma™ ABS233AF488

Catalog No. ABS233AFAMI

Description

Phosphatidylinositol 3-kinase regulatory subunit alpha (UniProt: Q63787; also known as PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha) is encoded by the Pik3r1 (Gene ID 25513) gene in rat. PI3 Kinase is a heterodimeric complex composed of an 85kDa regulatory subunit and a 110kDa catalytic subunit. It binds to activated (phosphorylated) protein tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. PI3 Kinase catalyzes the phosphorylation of PI(4,5)P2 (PIP2) inositol ring at the 3 position to generate PI(3,4,5)P3 (PIP3), a potent second messenger that mediates the action of several growth factors. PI3 kinase is essential for the insulin signaling and also plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Tyrosine phosphorylation of growth factor receptors creates docking sites for p85 binding (through its SH2 domains), bringing p85-associated p110 proximal to its lipid substrate on the membrane. PI3 Kinase is also activated by Ras and by the beta and gamma subunits of heterotrimeric G-proteins.

Specifications

• Antigen: PI3 Kinase p85, N-SH2 domain
• Classification: Polyclonal
• Conjugate: Alexa Fluor 488
• Gene: Pik3r1
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This polyclonal antibody targets the first SH2 domain region common to human (isoform 1, AS53, p46, p85I), mouse, and rat (isoform p85-alpha, p55-alpha, and p50-alpha) spliced isoforms of PI3 kinase regulatory subunit alpha reported by UniProt (P27986, P26450, Q63787).
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.

• Applications: Immunocytochemistry
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified rabbit polyclonal antibody Alexa Fluor™ 488 conjugate in PBS with 1.5% BSA and 0.05% Sodium Azide.
• Gene Accession No.: Q63787
• Immunogen: GST-tagged recombinant fragment corresponding to the first SH2 domain of rat PI3 kinase regulatory subunit alpha.
• Quantity: 100 μL
• Research Discipline: Signaling
• Gene ID (Entrez): NP_037137
• Target Species: Human

For Research Use Only. Not for use in diagnostic procedures.