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Thermo Scientific™ Pierce™ 3x DYKDDDDK Peptide
Description
Pierce 3X DYKDDDDK Peptide is a lyophilized peptide with the sequence Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (MDYKDHDGDYKDHDIDYKDDDDK). It has a molecular weight of 2861.9 g/mol. For use, reconstitute to 1.5 mg/mL in PBS.
This tricosa-peptide can be used to competitively elute DYKDDDDK-tagged fusion proteins from immobilized anti-DYKDDDDK affinity supports, especially when a DYKDDDDK-tagged protein is sensitive to low pH. At a 1.5 mg/mL concentration, complete release of tagged protein can be achieved in 5–10 minutes at ambient temperature.
Note: Pierce 3X DYKDDDDK Peptide may interfere with protein determination assays and absorbance at 280 nm. However, the peptide can be removed from the eluate by desalting (e.g., with Zeba Spin Desalting Columns, Cat. No. 89891).
Specifications
Specifications
| Product Line | Pierce™ |
| Product Type | Peptides |
| Protein Form | Recombinant |
| Quantity | 5 mg |
| Content And Storage | 5 mg lyophilized peptide. Store at -5 to -30°C. |
| Format | Lyophilized Powder |
| Shipping Condition | Wet Ice |
| For Use With (Application) | Protein Purification and Isolation |
| Form | Lyophilized Powder |
| Matrix | Affinity Resin |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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