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Thermo Scientific™ Pierce™ 6 Protein Digest, Equimolar, LC-MS grade PROMO
Description
The Thermo Scientific Pierce 6 Protein Digest Standard, Equimolar, LC-MS Grade, is a highly validated equimolar protein digest mixture for use as a quality control sample for mass spectrometry (MS) analysis of proteomic samples.
- Positive control sample - optimized and validated as a quality control standard for mass spectrometry applications
- Excellent sequence coverage - on average, greater than 70% sequence coverage of the 6-protein mixture
- Validated peptide quality - digestion procedure optimized for minimal missed cleavages and over-alkylation
- Rigorously tested - high-quality, consistent, 6-protein digest guaranteed and documented via lot-specific certificates of analysis
- Stable - provided in a stable lyophilized format
The Pierce 6 Protein Digest Standard contains an equimolar mixture of highly pure bovine cytochrome c, lysozyme, alcohol dehydrogenase, bovine serum albumin, apo-transferrin, and beta-galactosidase. The proteins are reduced, alkylated (iodoacetic acid), digested (MS-grade trypsin), and desalted (C18) after trypsin digestion to provide a quality LC/MS-grade standard free of intact protein. The peptide digest provides excellent sequence coverage with minimal over-alkylation and missed cleavages. This digest has been robustly tested for peptide quality, digestion efficiency, and lot-to-lot uniformity and is suitable for LC and LC-MS applications.
Specifications
Specifications
| For Use With (Equipment) | Mass Spectrometer |
| Product Type | Protein Digest |
| Product Line | Pierce™ |
| Content And Storage | Store in freezer (-5°C to -30°C). |
| Starting Material | Peptides |
| Final Product Type | Peptides |
| Detection Method | Mass Spectrometry |
| Workflow Step | Calibration |
| Quantity | 100 pmol |
| Form | Solid |
For Research Use Only. Not for use in diagnostic procedures.
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