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Thermo Scientific™ Pierce™ Antibody Biotinylation Kit for IP PROMO

Catalog No. PI90407PM
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Thermo Scientific™ Pierce™ Antibody Biotinylation Kit for IP
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Catalog No.
PI90407PM Thermo Scientific™ Pierce™ Antibody Biotinylation Kit for IP
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Catalog No. PI90407PM Supplier Thermo Scientific™ Supplier No. 90407

Description

Label primary antibodies with biotin for use in agarose or magnetic bead immunoprecipitation experiments using the Thermo Scientific™ Pierce™ Antibody Biotinylation Kit for IP.

The Thermo Scientific™ Pierce™ Antibody Biotinylation Kit for IP provides biotinylation reagents designed specifically for the labeling of primary antibodies used in immunoprecipitation applications.

  • Optimized for IP - reagents and protocols developed specifically to address antibody labeling for immunoprecipitation applications
  • Easy to use - kit configuration specifically optimized to accommodate 50 to 200μg of antibody in a 100μL reaction; desalting columns are provided to remove excess biotinylation reagent after labeling
  • Amine reactive - reacts with primary amines (-NH2) of antibodies
  • Enhanced solubility - pegylation improves water solubility of the biotinylated antibody and prevents aggregation upon storage
  • Reduced steric hindrance - longer spacer arm (29 angstroms) minimize steric hindrance when binding to avidin molecules

The kit contains sufficient reagents to label 50 to 200μg of antibody in 100μL reaction volumes for eight samples. The NHS-PEG4-biotin labeling reagent contains an amine-reactive N-hydroxysuccinimide ester (NHS) group and a water-soluble PEG4 spacer for optimal labeling and is provided in easy-to-use, single-use microtubes. Both the labeling efficiency of the biotinylation reagent and binding affinity of the labeled antibody have been validated using mouse monoclonal (IgG1, IgG2), rabbit polyconal, and rabbit monoclonal antibodies. Zeba™ Desalting Spin Columns are provided for easy and efficient removal of salts and excess biotin.

Applications:

  • IP and co-IP
  • IP and co-IP for mass spectrometry applications
  • Antibody attachment to streptavidin agarose and magnetic supports

Specifications

Product Type Antibody Biotinylation Kit
Quantity 8 Reactions
Content And Storage EZ-Link NHS-PEG4-Biotin, 8 x 0.5 mg, Zeba Spin Desalting Columns (7K MWCO), 8 x 0.5 mL, 20X Phosphate Buffered Saline, 15 mL Store in refrigerator 2°C to 8°C).
Labeling Scale 100 μg
Product Line Pierce
Label or Dye Biotin
Labeling Target Antibodies
Product Title
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