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Thermo Scientific™ Pierce™ Binding and Elution Buffers for IgG Purification Available on GSA/VA Contract for Federal Government customers only.

Ensure high-yield, nondenaturing antibody purification using IgG affinity purification supports with these gentle buffer solutions.

$52.40 - $367.50

Specifications

Target IgG
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Products 13
Catalog Number Mfr. No. Description Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Description Quantity Price Quantity & Availability  
PI21001 View Documents Thermo Scientific™
21001
Pierce Protein A IgG Binding Buffer 1L Each for $98.50
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PI21004 View Documents Thermo Scientific™
21004
Pierce IgG Elution Buffer 1L Each for $92.00
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PI21007 Available on GSA/VA Contract for Federal Government customers only. View Documents Thermo Scientific™
21007
Pierce Protein A IgG Binding Buffer 3.75L Each for $275.00
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PI21009 Available on GSA/VA Contract for Federal Government customers only. View Documents Thermo Scientific™
21009
Pierce IgG Elution Buffer 3.75L Each for $232.00
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PI21011 Available on GSA/VA Contract for Federal Government customers only. View Documents Thermo Scientific™
21011
Pierce Protein G IgG Binding Buffer 3.75L Each for $286.00
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PI21012 View Documents Thermo Scientific™
21012
Pierce Gentle Ag/Ab Binding Buffer, pH 8.0 3.75L Each for $264.50
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PI21013 View Documents Thermo Scientific™
21013
Pierce Gentle Ag/Ab Elution Buffer, pH 6.6 3.75L Each for $367.50
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PI21019 View Documents Thermo Scientific™
21019
Pierce Protein G IgG Binding Buffer 1L Each for $88.70
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PI21027 View Documents Thermo Scientific™
21027
Pierce Gentle Ag/Ab Elution Buffer, pH 6.6 500mL Each for $127.20
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PI21030 View Documents Thermo Scientific™
21030
Pierce Gentle Ag/Ab Binding and Elution Buffer Kit 200mL kit Each for $129.40
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PI54200 View Documents Thermo Scientific™
54200
Pierce Protein A/G IgG Binding Buffer 240mL Each for $52.40
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PI21020 View Documents Thermo Scientific™
21020
Pierce Gentle Ag/Ab Binding Buffer, pH 8.0 1L Each for $133.10
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PI21028 View Documents Thermo Scientific™
21028
Pierce IgG Elution Buffer, pH 2.0 1L Each for $88.90
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Description

Description

Thermo Scientific™ Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads.

These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.

The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.

Finally, the Mouse IgG1 Mild Elution Buffer, when used in combination with Protein A resin, enables selective elution and separation of the IgG1 subclass from total bound mouse IgG.

Highlights:

  • Three optimized binding buffers – for Protein A, Protein G or Protein A/G

  • Two elution buffer options – acidic (pH 2.8) or high salt (near-neutral)

  • Special system for mouse IgG1 – selective, two-stage elution from Protein A agarose

Item-Specific Features:

  • Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA

  • Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA

  • Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide

  • IgG Elution Buffer: pH 2.8; amine-based

  • Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide

  • Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)

  • Mouse IgG1 Mild Elution Buffer: pH 6.0; contains 0.02% sodium azide

Includes:

Kits contain both binding and elution buffers

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

Specifications

IgG
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