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Thermo Scientific™ Pierce™ CDI-activated Agarose Resin

Catalog No. PI20259
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Quantity:
10 mL

Description

Covalently immobilize N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions using carbonyldiimidazole-activated resin.

Thermo Scientific™ Pierce CDI-Activated Agarose is a porous, beaded chromatography support that is activated with carbonyldiimidazole groups for covalent immobilization of amine-containing affinity ligands.

Pierce CDI-activated Agarose is a porous, beaded chromatography support that is activated with carbonyl diimidazole groups for covalent immobilization of proteins and other molecules that contain N-nucleophiles. Coupling can be performed in aqueous or organic solvent conditions, making possible the immobilization of many kinds of ligands. Once a molecule is immobilized, the prepared affinity resin can be used multiple times in typical affinity purification procedures.

Highlights:

  • Reliable coupling chemistry – immobilization occurs through the reaction of N-nucleophiles with 1,1'-carbonyldiimidazole groups of the resin to form stable, uncharged N-alkylcarbamate linkages

  • Easy to use – no secondary reagents needed; just wash equilibrate resin in alkaline coupling buffer and add ligand; reaction proceeds spontaneously

  • Stable activation – half-life of hydrolysis is much longer than hydroxysuccinimide ester activations, making immobilization reactions easier to prepare and control; simplifies filtration and washing before adding a ligand or protein

  • Well-defined coupling condition – reaction is most efficient with primary amines at pH 9 to 11

Properties of CDI-Activated Agarose:

  • Resin: crosslinked 6% beaded agarose
  • Activation: at least 50μmol 1,1'-carbonyldiimidazole (CDI) groups per milliliter of resin
  • Format: supplied as stabilized, 50% slurry in acetone
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store at 4°C.
Product Line Pierce™
Quantity 10 mL
Target Primary Amine
Type Resin
Stationary Phase Carbonyl Diimidazole
Format Bottle
Column Type Affinity, Agarose Resin

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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