Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Pierce™ Copper Coated High Capacity Plates

Catalog No. p-7227134
Click to view available options
Color:
Black
Clear
White
Format:
Standard
Strip Plate
Detection Method:
Chemiluminescence
Colorimetric
Fluorescence

Thermo Scientific Pierce Copper Coated High Binding Capacity (HBC) Plates use an exclusive coating process to increase the amount of histidine-tagged protein that will bind to the plate surface.

Pierce Copper Coated Plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metals such as copper. Although copper is less discriminating in its binding specificity than Nickel, the higher binding capacity of these plates is ideal for high throughput screening applications that need improved sensitivity and greater dynamic range.

Thermo Scientific Pierce Copper Coated High Binding Capacity (HBC) Plates use an exclusive coating process to increase the amount of histidine-tagged protein that will bind to the plate surface.

Features of Copper Coated High Binding Capacity Plates include:
• Quantitate previously undetectable histidine-tagged proteins
• Wider dynamic range with four-fold greater capacity than regular nickel chelate-coated plate
• Copper chelate provides greater binding capacity than nickel
• The detection limit is 0.1 pg of polyhistidine tagged fusion protein per well
• Pre-blocked for the ultimate convenience

Note: Copper coated plates are for use with purified His-tagged proteins. Typical cell lysates contain non-his-tagged proteins that may bind to copper, which can reduce binding capacity and signal-to-noise ratios. For cell lysates, use Thermo Scientific Pierce Nickel Coated Plates.

TRUSTED_SUSTAINABILITY

Specifications

Binding Property High Capacity, Affinity
Format Standard
Content And Storage Store at 4°C in unopened pouches. Once opened, store unused plates in a resealable bag with desiccant at 4°C.
Detection Method Fluorescence
Packaging 5 Plates
Product Line Pierce
Color Black
Product Type Microplate
Material Polystyrene
No. of Wells 96
Surface Treatment Divalent Copper, Metal Chelate
Plate Blocking Blocker™ BSA Buffer
Quantity 5 plates
Show More Show Less
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.