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Thermo Scientific™ Pierce™ Crosslink Magnetic IP/Co-IP Kit PROMO
Description
An HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.
- Host Species: Mouse
- Cross Reactivity: Tag
- Isotype: IgG1
- Clone: 2-2.2.14
- Immunogen: HA peptide YPYDVPDYA derivitized to ovalbumin
- Formulation: 1 mL Purified Antibody in PBS, with 0.05% sodium azide; pH7.2
- Synonyms: human influenza hemagglutinin, HA-tag, HA, HA Epitope Tag
26181 is supplied as 1 mL of antibody on crosslinked 4% beaded agarose supplied as a 50% slurry in 0.1M phosphate, 0.15M NaCl, pH 7.2 with 0.05% sodium azide. Specific for the HA peptide YPYDVPDYA. The anti-HA agarose has been used successfully in immunoprecipitation applications. The anti-HA agarose has 3.5mg mouse anti-HA IgG1 monoclonal antibody conjugated permL of settled agarose resin. HA Synthetic Peptide (Product # 26184) is available for use in neutralization and control experiments and to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin.
Specifications
Specifications
| Content And Storage | Sufficient For: 40 IP Reactions using 25 μL of beads, Pierce Protein A/G Magnetic Beads, 1 mL, IP Lysis/Wash Buffer, 2 x 50 mL, Coupling Buffer (20X), 25 mL, DSS Crosslinker, 8 x 2 mg, Lane Marker Sample Buffer (5X), 5 mL, Elution Buffer, 25 mL, Neutralization Buffer, 1 mL, Lane Marker Sample Buffer (5X), 5 mL Store at 4°C. Store DSS desiccated at 4°C. |
| Assay | Affinity Purification |
| For Use With (Equipment) | KingFisher™ Flex Magnetic Particle Processor, Magnetic Stand |
| Product Line | Pierce |
| Quantity | 40 Reactions Kit |
| For Use With (Application) | Immunoprecipitation (IP) |
| Target | Not Target-Specific |
| Type | Immunoprecipitation/Co-Immunoprecipitation Kit |
| Sufficient For | 40 Reactions |
| Format | Kit |
For Research Use Only. Not for use in diagnostic procedures.
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