Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Pierce™ Gaussia-Firefly Luciferase Dual Assay Kit

Simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.

$199.60 - $1374.00

Specifications

Type Assay Kit
Target Luciferase, Firefly Luciferase, Gaussia Luciferase
Assay Reporter Enzyme, Luciferase Reporter Assay
Compatible Cells Mammalian Cells
Detection Method Bioluminescence
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
PI16181
View Documents
Thermo Scientific™
16181
100 Reaction Kit
Each for $199.60
Edge
Add to Cart
 
PI16182
View Documents
Thermo Scientific™
16182
1000 Reaction Kit
Each for $1,374.00
Add to Cart
 
Description

Description

The Thermo Scientific™ Pierce Gaussia-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.

The Pierce Gaussia-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Gaussia and Red Firefly luciferase activity. Gaussia luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. This reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.

Highlights:

  • Simultaneous – concurrent, filter-based, wavelength-separated detection of two luciferase activities

  • Sensitive – measure bright blue Gaussia and red firefly luciferase activities in the same sample

  • Fast – no quenching step required, unlike in traditional sequential dual assays

  • Multiplex – capable of quantitating two cellular activities in the same sample(s)

Includes:

Cell lysis buffer, reaction buffer, and substrates coelenterazine and D-luciferin

Requires:

Gaussia and Red Firefly luciferase reporters; Filter sets in the ranges of 425 to 525nm for Gaussia Luc and 615 to 675nm for Red Firefly Luc. Luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; Injector required for assessing more than 24 wells at a time.

Recommended for:

Study two regulatory elements at the same time; Monitor two signaling pathways simultaneously; Enable studying more than one target per screen (e.g. off-target effects)

Specifications

Specifications

Assay Kit
Reporter Enzyme, Luciferase Reporter Assay
Bioluminescence
384-well plate, 96-well plate
Chemical Substrate
Luciferase Substrate
Coelenterazine, D-Luciferin
Luciferase, Firefly Luciferase, Gaussia Luciferase
Mammalian Cells
Luminometer (Microplate)
Enzyme Labeled
Enhanced Chemiluminescence
Pierce™
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

Provide Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.