Thermo Scientific Pierce Glycoprotein Staining Kit
Rapidly stain glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method in this convenient kit.
Manufacturer: Thermo Scientific 24562
Thermo Scientific Pierce Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method.The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.
Specific for glycoproteins periodic acid-Schiff (PAS) method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups
Optimized protocol the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours (half the time required for other staining methods)
Colorimetric glycoproteins are detected as magenta bands against a faint pink or colorless gel background
Qualitative staining detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16g horseradish peroxidase per band
Controls included complete kit includes positive and negative control proteins (HRP and STI, respectively).
Gels and membranes effective for use with polyacrylamide mini gels or nitrocellulose membranes
Three reagents in 250mL bottles (1 solution and 2 dry powders to which water must be added), plus two vials of lyophilized proteins (1mg each)
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|In-Blot Detection, In-Gel Detection|
|Sufficient For: 10 mini gels (SDS-PAGE)
• Oxidation Reagent, 2.5 g powder to make 250 mL
• Reduction Reagent, 1.25 g powder to make 250 mL
• Positive Control (Horseradish Peroxidase), 1 mg
• Negative Control (Soybean Trypsin Inhibitor), 1 mg
• Glycoprotein Stain, 250 mL
Store all components at 4°C.
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