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Thermo Scientific™ Pierce™ HA-Tag IP/Co-IP Kit
Description
Thermo Scientific™ Pierce HA Tag IP and Co-IP Kit provides the affinity resin and other reagents necessary to easily perform immunoprecipitation (IP) or co-immunoprecipitation (co-IP) reactions using an HA-tagged protein as the bait.
With sufficient materials to perform 25 IP or Co-IP assays, the complete HA Tag IP/Co-IP Kit includes the immobilized anti-HA antibody on agarose resin, binding, wash and elution buffers, microcentrifuge spin columns, HA-tag positive control lysate, sample loading buffer and easy-to-follow instructions.
Highlights:
- Improved updated kit includes more immobilized antibody resin and improved protocol along with GST-PI3K-SH2-HA fusion protein as a positive control
- Specific highly specific anti-HA monoclonal antibody provides high-yield immunoprecipitation products and clean Western blot detection
- Compatible includes protocols and reagents for multiple elution conditions to accommodate different protein sensitivities and downstream applications
- Robust the kit and the anti-HA agarose are compatible with various cell lysates and physiologic (non-denaturing) buffer systems
- Convenient and Easy complete kit includes all necessary reagents, convenient spin columns and easy-to-follow instructions
Includes:
Anti-HA agarose resin, TBS pack, elution buffer, lane marker nonreducing sample buffer, positive control lysate, microcentrifuge spin columns and collection tubes
Recommended for:
Purification and detection of HA-tagged fusion-protein for downstream assays; Enrichment and screening of HA-tagged fusion-protein mutants; Co-immunoprecipitation and screening of interacting proteins
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Specifications
Specifications
| Content And Storage | Sufficient For: 25 IP/Co-IP reactions, each using 10 μL of resin • Anti-HA Agarose, 0.25 mL • HA-tagged Positive Control, 0.5 mL • BupH Tris Buffered Saline Pack (500 mL), 1 pack • Lane Marker Non-Reducing Sample Buffer (5X), 5 mL • Pierce Spin Columns Accessory Pack, 27 columns • Collection Tubes and Caps Accessory Pack, 100 tubes Store HA-tagged positive control at -80°C (may be stored at -20°C for up to 6 months). Store remaining kit components at 4°C. |
| Assay | Affinity Purification |
| For Use With (Equipment) | Microcentrifuge |
| Product Line | Pierce™ |
| Quantity | 25 reactions kit |
| For Use With (Application) | Pull-Down (Tag-Based), Immunoprecipitation (IP) |
| Target | Target-Specific |
| Type | Tag IP/Co-IP Kit |
| Sufficient For | 25 Reactions |
| Format | Kit |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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