Thermo Scientific™ Pierce™ HA-Tag Magnetic IP/Co-IP Kit

Description

Perform immunoprecipitation (IP) assays of HA-tagged proteins or co-IP reactions with HA-tagged bait proteins with these magnetic beads, positive control and reagents.

Perform immunoprecipitation (IP) assays of HA-tagged proteins or co-IP reactions with HA-tagged bait proteins with the magnetic beads, positive control and reagents provided in the Thermo Scientific™ Pierce Magnetic IP/Co-IP Kit.

Additional Information and Online Ordering

The complete Magnetic IP/Co-IP Kit can be used to perform 40 IP or Co-IP assays. It includes magnetic beads coated with immobilized anti-HA antibody, lysis/wash buffer, low pH elution buffer, neutralization buffer, HA-tag positive control lysate, and non-reducing sample buffer. Protocols are provided for both manual and automated workflows.

Highlights:

• Specific – highly specific anti-HA monoclonal antibody enables high yields of immunoprecipitation products
• Fast – Optimized protocol is completed in approximately 1 hour
• Low non-specific binding – stable, pre-blocked beads provide highly purified HA-tagged proteins
• Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific™ KingFisher Instruments)
• Convenient and easy – complete kit includes all necessary reagents and easy-to-follow instructions

Includes:

The kit contains magnetic beads immobilized with anti-HA antibody, lysis/wash buffer, low pH elution buffer, neutralization buffer, HA-tag positive control lysate, and non-reducing sample buffer.

Recommended for:

High-throughput immunoprecipitation of recombinant HA-tagged proteins and co-IP of interacting proteins

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Specifications

• Capacity (Metric): 1 mL
• Formulation: Slurry: 10 mg/mL, 1% solids
• Content And Storage: Sufficient For: 40 IP reactions using 25 μL of anti-HA magnetic beads
  • HA-tagged Positive Control (Part No. 26180X), 500 μL
  • Pierce Anti-HA Magnetic Beads, 1 mL
  • Pierce IP Lysis/Wash Buffer, 2 x 50 mL
  • Non-Reducing Sample Buffer (5X), 5 mL
  • Elution Buffer (pH 2.0), 5 mL
  • Neutralization Buffer (pH 8.5), 1 mL
  
  HA-tagged Positive Control ships separately—store at -70°C. Store remaining components at 4°C.
• Assay: Affinity Purification
• For Use With (Equipment): KingFisher™ Flex, Magnetic Stand
• Product Line: Pierce™
• Quantity: 40 reactions
• For Use With (Application): Pull-Down (Tag-Based), Immunoprecipitation (IP)
• Target: Target-Specific
• Type: Tag IP/Co-IP Kit
• Sufficient For: 40 Reactions
• Format: Kit

For Research Use Only. Not for use in diagnostic procedures.