Thermo Scientific™ Pierce™ IgM Purification Kit PROMO

Description

Easily purify IgM from mouse ascites fluid using immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.

Pierce MBP Agarose is purified mannan binding protein (also called mannose-binding lectin, MBL) that has been immobilized on 4% beaded agarose. When used with an optimized buffer system based on temperature- and calcium-dependent binding, this resin enables specific affinity purification of mouse IgM from ascites fluid and other concentrated samples. The resin and essential buffers are available individually or bundled together in a complete IgM Purification Kit containing a pre-packed 5 mL column of the MBP Agarose.

Highlights:

• Immobilized MBP – affinity system based on mannan binding protein (MBP) allows specific purification of mouse IgM from ascites fluid or partial isolation of human IgM from serum

• Complete kit – IgM Purification Kit includes a pre-packed MBP column and ready-to-use buffers for convenient purification of at least 1.5mg IgM from 0.5 mL ascites samples per column-use

• Mild conditions – nondenaturing, near-neutral buffer conditions ensure recovery of functional antibodies

• Reusable – stable ligand and resins can be regenerated and reused at least 10 times without significant decline in binding capacity

Includes:

Kit contains a 5 mL MBP Agarose Column, column preparation buffer, IgM binding and elution buffers, and a column extender

Specifications

• Product Type: IgM Purification Kit
• Product Line: Pierce™
• Column Type: Agarose Resin, Affinity
• Format: Kit
• Stationary Phase: Lectin
• Purification Target: IgM Antibody
• Content And Storage: Sufficient For: 10 IgM purifications, each from 0.5 mL mouse ascites samples, MBP Agarose Column, 5 mL, 1 column, IgM Binding Buffer, 800 mL, IgM Elution Buffer, 500 mL, MBP Column Preparation Buffer, 50 mL, Column Extender Store at 4°C
• Quantity: 1 Column

For Research Use Only. Not for use in diagnostic procedures.