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Thermo Scientific™ Pierce™ Iminobiotin Agarose PROMO
Description
Pierce Biotin Agarose and Iminobiotin Agarose are beaded agarose supports onto which biotin and iminobiotin moieties have been covalently immobilized. Typical avidin-biotin affinity procedures use immobilized avidin or streptavidin proteins to capture biotin-tagged molecules from a sample. Occasionally, however, the reverse situation arises in which immobilized biotin in needed to capture avidin or streptavidin components from a sample. These biotin and iminobiotin resins address this special situation.
Immobilized biotin binds avidin and streptavidin very strongly, making it appropriate for non-reversible binding applications. Immobilized iminobiotin is the guanido analog of biotin. The dissociation constant of the avidin-iminobiotin complex is pH dependent. At pH 9.5 to 11, the avidin-iminobiotin complex will bind tightly. At pH 4, the avidin-iminobiotin complex will dissociate. Thus, iminobiotin agarose can be used for situations requiring reversible binding and elution of avidin components.
Highlights:
- Enable purification or removal of avidin or streptavidin samples
- Resins are crosslinked beaded agarose onto which biotin or iminobiotin are covalently immobilized
- Use Biotin Agarose for non-reversible binding (e.g., removal of avidin components from a sample)
- Use Iminobiotin Agarose for reversible binding (e.g., non-denaturing purification of avidin)
Specifications
Specifications
Content And Storage | Upon receipt store at 4°C. |
Product Line | Pierce™ |
Quantity | 5 mL |
Target | Streptavidin |
Type | Agarose |
Stationary Phase | Biotin Derivative |
Format | Bottle |
Column Type | Affinity, Agarose Resin |
For Research Use Only. Not for use in diagnostic procedures.
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