Thermo Scientific™ Pierce™ Iminobiotin Agarose PROMO

Description

Agarose beads of immobilized biotin (and iminobiotin) to strongly (or reversibly) bind and remove (or purify) streptavidin or avidin proteins and conjugates from samples.

Pierce Biotin Agarose and Iminobiotin Agarose are beaded agarose supports onto which biotin and iminobiotin moieties have been covalently immobilized. Typical avidin-biotin affinity procedures use immobilized avidin or streptavidin proteins to capture biotin-tagged molecules from a sample. Occasionally, however, the reverse situation arises in which immobilized biotin in needed to capture avidin or streptavidin components from a sample. These biotin and iminobiotin resins address this special situation.

Immobilized biotin binds avidin and streptavidin very strongly, making it appropriate for non-reversible binding applications. Immobilized iminobiotin is the guanido analog of biotin. The dissociation constant of the avidin-iminobiotin complex is pH dependent. At pH 9.5 to 11, the avidin-iminobiotin complex will bind tightly. At pH 4, the avidin-iminobiotin complex will dissociate. Thus, iminobiotin agarose can be used for situations requiring reversible binding and elution of avidin components.

Highlights:

• Enable purification or removal of avidin or streptavidin samples
• Resins are crosslinked beaded agarose onto which biotin or iminobiotin are covalently immobilized
• Use Biotin Agarose for non-reversible binding (e.g., removal of avidin components from a sample)
• Use Iminobiotin Agarose for reversible binding (e.g., non-denaturing purification of avidin)

Specifications

• Content And Storage: Upon receipt store at 4°C.
• Product Line: Pierce™
• Quantity: 5 mL
• Target: Streptavidin
• Type: Agarose
• Stationary Phase: Biotin Derivative
• Format: Bottle
• Column Type: Affinity, Agarose Resin

For Research Use Only. Not for use in diagnostic procedures.