Thermo Scientific™ Pierce™ Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit PROMO

Description

Efficiently digest mouse IgG1 antibodies into Fab or F(ab')2 fragments with immobilized ficin protease.

The kits use ficin protease to enzymatically digest the Fc portion of whole IgG to yield Fab or F(ab')₂ fragments. Because the ficin protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free. An optimized procedure and high-performance desalting and Protein A spin columns make these kits easy to use.

Highlights:

• Enzyme-free digestion products – immobilized ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease

• Optimized for mouse IgG₁ – the kit procedure is specifically optimized for monoclonal mouse IgG₁ antibodies, providing the best possible yield for this antibody subclass

• High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time. Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG

• Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments

Includes:

Ficin agarose resin, cysteine, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories

Specifications

• Quantity: 10 Antibody Samples
• Product Line: Pierce™
• Content And Storage: Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG, Pierce Ficin Agarose, 0.8 mL, Cysteine-HCl, 1 g, Mouse IgG1 Digestion Buffer, 120 mL, NAb Protein A Plus Spin Columns, 0.2 mL, 2 Columns, Protein A Binding Buffer, 50 mL, IgG Elution Buffer, 50 mL, Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 Columns, Spin Columns, 0.8 mL, 10 Columns, Microcentrifuge tubes, 2 mL, 30 Tubes, Store at 4–8°C.

For Research Use Only. Not for use in diagnostic procedures.