Thermo Scientific Pierce NHS-Activated Magnetic Beads enable covalent, amine-based conjugation of proteins to magnetic beads in a simple mix-and-go format for use in custom affinity purification experiments.The activated magnetic beads contain N-hydroxysuccinimide (NHS) functional groups that react with primary amines forming stable amide linkages. Once they are covalently attached, the immobilized proteins are highly resistant to leaching from the bead surface. When prepared beads are used in experiments, nonspecific binding is negligible because nonreacted NHS-ester groups are thoroughly blocked during the coupling procedure. Pierce NHS-Activated Magnetic beads can be coupled and processed either manually with a magnetic stand or with automated platforms such as the Thermo Scientific KingFisher Instruments.
High capacity at least four times higher binding capacity than NHS-activated magnetic beads from other suppliers
Easy to use immobilize in a simple one-step reaction with minimal hands-on time
Safe no hazardous chemicals needed (e.g., sodium cyanoborohydride and cyanogen bromide)
Ligand-compatible use to immobilize with nearly any primary amine-containing compound or affinity ligand
Low non-specific binding the bead surface is pre-blocked and any nonreacted NHS-ester groups are fully quenched
Protocol-compatible protein coupling to the beads and downstream applications can be performed manually or by automation (e.g., Thermo Scientific KingFisher Instruments)
Immobilization of ligands for the purification of recombinant proteins; Immobilization of antibodies for immunoprecipitations and co-immunoprecipitations free of antibody contamination in the eluates; Immobilization of secondary antibody for the affinity purification of antibody subtypes from ascites, serum and cell culture supernatant
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