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Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads

Thermo Scientific™ Pierce™ Ni-NTA Magnetic Agarose Beads provide a fast, convenient method for purification of His-tagged recombinant proteins

$322.00 - $1208.00

Specifications

Shipping Condition Wet Ice
Form Suspension
Product Line Pierce™
Protein Form Recombinant
Type Magnetic Agarose Bead
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
PI78605
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Thermo Scientific™
78605
4 mL
Each for $322.00
Add to cart
 
PI78606
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Thermo Scientific™
78606
20 mL
Each for $1,208.00
Add to cart
 
Description

Description

Product features: 
Ni-NTA Magnetic Agarose Beads are used for small-scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). Tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates such as those prepared using Thermo Scientific B-PER Bacterial Protein Extraction reagents. Purification of His-tagged proteins is achieved using an NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites that allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Agarose Beads has been optimized to allow for high purity of the isolated His-tagged protein. 

  • High capacity—sufficient for both routine and demanding purification procedures; binds >70 mg of 6xHis-tagged protein per mL of settled resin
  • High-performance and reproducible beads—non-aggregating, magnetite (Fe3O4), superparamagnetic beads provide exceptional uniformity for both manual and automated HTS purification applications
  • Low nonspecific binding—  optimized purification protocol results in  better tagged-protein purification
  • Versatile—purify proteins using native or denaturing conditions
  • Compatible—use with Thermo Scientific Cell Lysis reagents and a variety of buffer additives

Applications:
  • Protein purification and isolation 
  • High throughput protein purification using automated magnetic particle processor
Specifications

Specifications

Wet Ice
Pierce™
Magnetic Agarose Bead
Bottle
Suspension
Recombinant
Metal Chelate, Divalent Nickel
SDS
Product Certifications
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