Thermo Scientific™ Biodyne™ B Nylon Membrane, 0.45 μm, 8 cm x 12 cm

Catalog No.	Quantity
PI77016	25 sheets

Catalog No. PI77016 Supplier Thermo Scientific™ Supplier No. 77016

Description

Sheets of unmodified or positively charged Biodyne A and Biodyne B Nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection.

Thermo Scientific™ Biodyne A and Biodyne B Nylon Membranes are sheets of unmodified or positively charged Nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection methods.

These two varieties of nylon membrane are manufactured with a similar matrix pore size (0.4 micron), and both are supplied in packages of 8 x 12cm sheets. The Biodyne A Membrane contains both negatively and positively charged groups, enabling it to bind many kinds of macromolecules. The Biodyne B Membrane contains exclusively positive charges, providing the highest possible binding capacity for negatively charged molecules like DNA and RNA. The membranes are resistant to heat and solvents and will not shrink, crack or tear.

Highlights:

• Versatile and durable – achieve strong DNA binding by baking or UV-crosslinking without damaging the membrane

• Easy to use – pre-cut 8 x 12cm sheets are the ideal size for standard mini-gel transfers and microplate dot-blots

• High signal-to-noise ratio – lower background than nitrocellulose and most other nylon transfer membranes

Biodyne A Nylon Membrane

• Offers superior signal-to-noise ratios resulting in high sensitivities Provides extremely low backgrounds
• Contains 50% amino and 50% carboxyl groups
• Provides a hydrophilic surface with an isoelectric point at pH 6.5

Biodyne B Nylon Membrane

• Surface is modified with Positive Zeta Potential Provides high sensitivities
• Gives rapid nucleic acid binding without UV light or baking
• Enhances attraction of negatively charged biological molecules

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Specifications

• Length (Metric): 12 cm
• Width (Metric): 8 cm
• Quantity: 25 sheets
• Format: Sheet
• Material: Nylon
• Pore Size: 0.45 μm
• Content And Storage: Note: Always wear gloves when handling membranes because oils from fingers may prevent proper wetting. Also, proteins and other contaminants from hands may bind to the membranes and cause background.
• Dimensions (LxW): 12 cm x 8 cm
• Product Line: Biodyne

Frequently Asked Questions (FAQs)

Is your Biodyne B Nylon Membrane supplied sterile?

No, our Biodyne B Nylon Membrane is not supplied sterile.

What is the pore size of your Biodyne B Nylon Membrane?

The pore size of our Biodyne B Nylon Membrane is 0.45 µm.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.