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Thermo Scientific™ Pierce™ Protein L Plus Agarose PROMO
Description
Pierce Protein L Plus Agarose is a high-capacity affinity resin for purification of mammalian IgG isotypes that contain specific kappa light chains. Protein L is especially suited for isolating mouse IgG VkI and human IgG VkI, VkIII and VkIV monoclonal antibodies. The resin consists of recombinant Protein L immobilized on crosslinked 6% beaded agarose (CL-6B) using a stable, leach-resistant chemistry. The agarose beads can be regenerated and reused multiple times when stored properly.
Highlights:
Protein L – immobilized Protein L is ideal for selective purification of human and mouse antibodies that have kappa light chains
Agarose resin – support is crosslinked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
Inert and stable – superior manufacturing method immobilizes Protein L by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
High capacity – Plus variety of Pierce Protein L Agarose has a dense load of immobilized Protein L, providing a binding capacity of 10 to 20mg human IgG/mL resin
Properties of crosslinked 6% beaded agarose (CL-6B):
- Support pH Stability: 2 to 14 (short term); 3 to 13 (long term)
- Average Particle Size: 45 to 165 microns
- Exclusion Limit: 10,000 to 4,000,000 daltons
- Maximum Linear Velocity: 30cm per hour
- Maximum Pressure: less than 25psi (1.5 bar), defined as the maximum pressure drop across a column that the resin can withstand (Note: The indicated gauge pressure of a liquid chromatography apparatus may be measuring the total system pressure rather
- than the pressure drop across the column.)
Specifications
Specifications
Product Line | Pierce™ |
Column Type | Agarose Resin, Affinity |
Format | Bottle |
Stationary Phase | Protein L |
Purification Target | Ig Antibodies |
Content And Storage | Upon receipt store product at 4°C to 8°C. Product is shipped at ambient temperature. |
Quantity | 2 mL |
For Research Use Only. Not for use in diagnostic procedures.
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