The Thermo Scientific Pierce Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.This Flash Assay Kit contains reagents for measuring the activity of Renilla luciferase in mammalian cell lysates. When used with Thermo Scientific Green Renilla Luc Plasmids, the kit provides an extremely sensitive bioluminescent reporter assay system for intracellular detection of promoter or pathway activity. The signal produced by Green Renilla Luc is considerably greater than signal from either native firefly or Renilla luciferases assayed under similar conditions. Light output captured using a luminometer can be correlated with the amount of Renilla luciferase protein produced and used to determine the activity of the promoter driving Renilla expression.
Sensitive greater sensitivity allows utilization of smaller numbers of cells
Cost effective highest sensitivity assays result in decreased reagent consumption
Time-saving assays require minimal sample handling
Automation-friendly amenable to high throughput screens
Convenient contains a universal cell lysis buffer and optimized flash assay reagent
Safe allows one to perform non-radioactive assays
Cell lysis buffer, reaction buffer and substrate
Renilla luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers
Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies
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