Thermo Scientific™ Pierce™ RNA 3' End Biotinylation Kit PROMO

Description

Biotinylate 3-prime terminal ends of RNA probes for non-radioactive EMSA and other nucleic acid detection methods.

The complete kit contains an unlabeled RNA strand to use as a positive control, a biotinylated-RNA probe standard, RNase inhibitor, glycogen and ligation-enhancing reagents. The RNA 3' End Biotinylation Kit uses T4 RNA ligase to conjugate a single nucleotide analog to the 3' terminus of an RNA strand. The unique biotinylated cytidine (bis)phosphate used in this kit enables T4 RNA ligase to attach a single biotin tag on RNA probes of diverse secondary structure and lengths with greater than 70% efficiency.

Highlights:

• Non-radioactive – incorporates a biotin label which allows detection sensitivity similar to radioactivity when used with chemiluminescent detection systems

• Fast – RNA can be labeled in 0.5-2 hours with minimal downstream processing

• Easy to use – RNA ligase and optimized reaction buffer are included

• Economical – only a fraction of the cost of purchasing synthetic biotinylated RNA probes

• End-labeled – results in minimal disturbance of RNA secondary structure

• Flexible – label synthetic or in vitro transcribed RNA probes ranging from 22 to 450 nucleotides

Specifications

• Content And Storage: Sufficient For: 20 labeling Reactions (each with 50 pmol RNA), T4 RNA Ligase Reaction Buffer (10X), 100 μL, T4 RNA Ligase (20,000 U/mL), 40 μL, Non-labeled RNA Control (10 μM), 100 μL, Biotinylated IRE RNA Control (125 nM), 35 μL, RNase Inhibitor (40 U/μL), 2 x 10 μL, Nuclease-free Water, 1.5 mL, DMSO, 200 μL, 30% PEG, 300 μL, Biotinylated Cytidine (Bis)phosphate (1mM), 40 μL, Glycogen (20 mg/mL), 20 μL Store all components at -20°C
• Format: Kit
• Product Line: Pierce™
• Product Type: RNA Labeling Kit
• Quantity: 20 Reactions

For Research Use Only. Not for use in diagnostic procedures.