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Thermo Scientific™ Pierce™ Sulfo-SANPAH, No-Weigh™ Format

Catalog No. PI22589
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50 mg
10 x 1 mg
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PI22589 50 mg
PIA35395 10 x 1 mg
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Catalog No. PI22589 Supplier Thermo Scientific™ Supplier No. 22589
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Description

Thermo Scientific™ Pierce Sulfo-SANPAH is a long-arm (18.2 Å) crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.

Highlights:

  • Optimal photolysis occurs at 320-350nm, which limits damage to biomolecules by irradiation
  • Water-soluble
  • Reactive groups: sulfo-NHS ester and nitrophenyl azide (Amax= 470nm)
  • Reactive toward: amino groups and various sites
TRUSTED_SUSTAINABILITY

Specifications

Chemical Reactivity Amine-Nonselective
Cleavable By Thiols
Molecular Weight (g/mol) 492.4
PEGylated No
Spacer Arm Length 18.2 Å
Content And Storage Upon receipt store at -20°C protected from light and moisture.
Cell Permeability No
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety Sulfo-NHS Ester, Aryl Azide
Spacer Medium (10 to 30 Å)
Form Powder
Quantity 50 mg
Solubility Water
Format Standard, Single-use
Water Soluble Yes
Product Type Crosslinker
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Frequently Asked Questions (FAQs)

Can you provide the shelf-life for Sulfo-SANPAH?

Sulfo-SANPAH is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
What are the reactive groups on Sulfo-SANPAH and which groups do they react with?

Sulfo-SANPAH is a heterobifunctional crosslinker that has a sulfo-NHS ester and a photoactivatable nitrophenyl azide group. The sulfo-NHS ester reacts with primary amines in pH 7-9 buffers to form stable amide bonds. When exposed to UV light, nitrophenyl azides form a nitrene group that can initiate addition reactions with double bonds, insertion into C-H and N-H sites, or subsequent ring expansion to react with a nucleophile (e.g., primary amines).

For Research Use Only. Not for use in diagnostic procedures.

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